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Cell culture and treatment of NRK-52E cells NRK-52E cells, immortalized rat proximal tubular epithelial cells, were maintained in DMEM supplemented with 5% fetal bovine serum (FBS), 100 U/ml penicillin, 100 mg/ml streptomycin, and 26 mM NaHCO 3 , and were grown at 37 C in humidified 5% CO 2 in air

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