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Primary ESCs were seeded into 12-well plates and cultured until they reached 6080% confluency, followed by transfection with 20 pmol STAT6 siRNAs per well using the Lipofectamine RNAiMAX reagent (Invitrogen, California, USA) in Opti-MEM (Gibco, New York, USA) according to the manufacturers instructions

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A 2006 systematic review and meta-analysis concluded that such supplementation was associated with a 22% lower risk of cardiovascular defects in case-control studies and a 39% lower risk in cohort studies and randomized controlled trials (53)

Although certain indicators have been associated with the development of BIPN in clinical studies, genetic variations in drug response must also be taken into account