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Binding assays showed that 5mTHF failed to bind GNMT R241A, indicating that R241 is a critical and previously unrecognized residue within the 5mTHF-binding pocket ( Figure 4F )

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Nigella sativa has widespread pharmacological and therapeutic effects, including anticancer, antioxidant, hepatoprotective (16), anti-inflammatory (17), and renal protective activities (18)