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28 A study on the nanoparticle optimization of Nigella sativa reported the successful development and characterization of a nano-based carrier for Nigella sativa essential oil using optimization techniques, and a methyl thiazolyl-diphenyl-tetrazolium bromide (MTT) assay was performed to compare the in vitro cytotoxicity using two different cell lines (i.e., HCT 116 for colorectal carcinoma and PC3 for prostatic cancer)

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Specifically, 10-30 M MT significantly enhanced root system vigor, root hair growth, and lateral root development through root irrigation (Tian et al., 2024)

Interestingly, despite its widespread distribution in various tissues, GA exhibits a relatively low content in brain tissue (Ma et al., 2016)