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For quantitative reverse transcription PCR (RTqPCR), 1,000 ng of total RNA was reverse transcribed into complementary DNA (cDNA) using a Hifair II 1st Strand cDNA Synthesis Kit (Yeasen, China) following the manufacturers instructions

Minor side effects uch as transient coughing and an unpleasant odor are common with this treatment Are there any risks

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When homocysteine levels are too low, it suggests that traffic is being diverted heavily in one direction, potentially leaving other pathways undersupplied