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Detecting variant effects on fitness using TileSeq For each plasmid library, the tiling PCR was performed in two steps: (i) the targeted region of the ORF was amplified with primers carrying a binding site for Illumina sequencing adaptors and (ii) each first-step amplicon was indexed with an Illumina sequencing adaptor in the second-step PCR

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Twodimensional biomaterials applied as functional bioplatforms and devices: revolutionizing biomedical applications

SOD1 of Sonneratia alba and Rosa roxburghii , both derived from plant, had 70% activity after incubation at 55 C and 70 C for 1 h, respectively