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Further validation of the molecular mechanisms was performed using flow cytometry, Western blotting, and reverse transcription quantitative polymerase chain reaction (RT-qPCR)

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During the titration phase, the dosage is increased by 2.5 mg increments every 4 weeks

Clinical improvement was also observed in oxidative stress markers, with improvements in methionine levels (p=0.05), homocysteine levels (SAH, p=0.007) and the ratio of S-adenosylmethionine (SAMe) to SAH (p=0.007), indicating improvement with methylation at the cellular level and a decrease in oxidative stress in the body 2