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As two-photon imaging must be conducted in the dark where the pupil would be fully dilated, we illuminated the mouse eye contralateral to the recording side using 470 nm blue light fiber, with a shielding to avoid light contamination, to slightly constrict the pupil

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Sarkissian never convinces his patients to replace amalgam fillings unless there is evidence of failure or recurrent decay, or the patient prefers to replace them for cosmetic purposes