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Nat Clin Pract Oncol 5: 194204 Stoeltzing O, Liu W, Reinmuth N, Fan F, Parikh AA, Bucana CD, Evans DB, Semenza GL, Ellis LM (2003) Regulation of hypoxiainducible factor1alpha, vascular endothelial growth factor, and angiogenesis by an insulinlike growth factorI receptor autocrine loop in human pancreatic cancer

Short hairpin RNA (shRNA)-resistant (r) human rDLD-WT, rDLD-K430R, and rDLD-K430Q were made by introducing missense mutations at K430 and synonymous mutations in shRNA-targeting sites in PCR-amplified DLD and cloned into pLenti-EGFP-F2A-Hygro-CMV-MCS-WPRE vectors from OBio Biotechnology

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Hepatocellular carcinoma (HCC) is the most common type of liver cancer, accounting for ~90% of cases