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From this list, three top candidates were selected for evaluation in mice using the ex vivo mycobacterial growth inhibition assay (MGIA), with one antigen, metE, emerging as a promising candidate due to its strong immunogenic profile

Shaking the vial can denature the peptide, meaning its complex protein structure is damaged and it may lose its biological activity

Nambiar PR, Jackson ML, Ellis JA, Chelack BJ, Kidney BA, Haines DM (2001): Immunohistochemical detection of tumor suppressor gene p53 protein in feline injection site-associated sarcomas