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Meanwhile, western blot results indicated that the vitamin B12-treated groups showed reduced levels of cleaved-caspase12 expression relative to their untreated counterparts in Figure 2B,C

Key methodological innovations included (1) peptide conjugation to modulate Toll-like receptor 5 (TLR5) and Notch-1 signaling pathways, mimicking interactions with Bacillus subtilis flagellin, and (2) comprehensive validation across multiple models, including cell lines, patient-derived cells, organ-on-chip systems, murine models, and porcine studies

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