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CJC 1295 No Dac (5mg) + Ipamorelin (10mg) Blend Calculation: One pen contains 300 units of 50mcg (16.7mcg CJC No DAC and 33.3 mcg Ipamorelin) Examples of calculated units per pen of CJC 1295 No DAC (5 mg) + Ipamorelin (10 mg) BLEND corresponding to micrograms: 1 unit = 16.7 mcg CJC No DAC and 33.3 mcg Ipamorelin

After removing the magnetic beads, samples were spun down at 20,000 g for 10 min to remove the remaining beads, and the supernatant was transferred to a scintillation vial with 5 mL of Insta-Gel Plus scintillation cocktail (Perkin Elmer #601339)

doi: 10.1021/acs.jmedchem.8b01299 76

Protein binding may be decreased in the elderly resulting in a larger proportion of the total serum theophylline concentration in the pharmacologically active unbound form