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Platting Supplement kit A (FBS + Dexamethasone + Penicillin/Streptomycin + Human Insulin + Glutamax + HEPES buffer) (Gibco/Invitrogen, USA) was added to 500 ml of DMEM

The major methyltransferases are: COMT (catechol-O-methyltransferase) : inactivates catecholamines (dopamine, norepinephrine, epinephrine), catechol estrogens, and catechol drugs (L-DOPA) R HNMT (histamine N-methyltransferase) : the primary intracellular histamine degradation enzyme in the brain and peripheral tissues R INMT (indolethylamine N-methyltransferase) : methylates tryptamine, serotonin, and other indoleamines R NNMT (nicotinamide N-methyltransferase) : methylates nicotinamide (vitamin B3) and other pyridines R TMT (thiol S-methyltransferase) : S-methylates thiol drugs (captopril, D-penicillamine) and thiol metabolites R TPMT (thiopurine S-methyltransferase) : methylates thiopurine drugs (azathioprine, 6-mercaptopurine) used in autoimmune disease and cancer R Methylation capacity depends on the methylation cycle: methionine to SAM-e to SAH to homocysteine, recycled back to methionine by methionine synthase (B12-dependent) or BHMT (betaine-dependent)

Li WJ, Feng GS, Gauthier JM, Lokshina I, Higashikubo R, Evans S, et al

Among the enzymatic systems involved in the maintenance of the intracellular redox balance, a main role is played by GSH [8] that participates, not only in antioxidant defense systems, but also in many metabolic processes [9]