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Other notable techniques include plasma-free amino acid dose-response (Whitehouse et al., 2017), in situ and in vitro methods (Estes et al., 2022), and fecal amino acid excretion (Risnen et al., 2020), all of which are employed to evaluate rumen-protected amino acids

L2-Cmu development and validation To target IGF-1R action in mice, we utilized the mAb, L2-Cmu (Amgen Inc, Thousand Oaks, CA), which is a murinized IgG1 version of the fully human L2-C mAb previously reported by Calzone et al