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T.BrosnanM

The mechanisms influenced and regulated to drive changes in each population differ

If it is more than 0.1 or 0.2 pH units away from your desired pH, then youll want to use concentrated NaOH to adjust the pH of your elution buffer

In vitro studies confirm minimal cytotoxicity with no impact on cell viability up to 100 M concentrations across multiple cell types, though higher concentrations may produce non-specific effects that could confound experimental results