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(PubMed) Karanikas G, Schuetz M, Kontur S, et al

PMID: 24425802

The limited library size relative to the vast sequence length means that many rounds of selection are unavoidable for protein optimization

Potential mechanisms include: Alterations in methylation pathways Changes in NAD+ availability Mild dehydration secondary to metabolic shifts Headaches are generally mild but may become persistent in sensitive individuals