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In parallel, 336 KOs were found to be inversely proportional to the BWG, including those encoding for bacterial biosynthesis of cofactors and amino acids (e.g., phenylalanine, tyrosine, tryptophan, histidine, and lysine), carbon and pyruvate metabolism (including glycolysis/gluconeogenesis, galactose metabolism, and carbon fixation), together with the metabolism of nucleotides (including DNA replication and homologous recombination) and the metabolism of starch and sucrose and fructose and mannose

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The first breakpoint occurs at the downregulated isocitrate dehydrogenase (IDH) 1, which converts isocitrate into -ketoglutarate (-KG) in M2, resulting in citrate accumulation, facilitating the biosynthesis of fatty acids and prostaglandins in M1 macrophages [12]