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The overall total cellular amino acid concentrations were altered in the ATP6AP1 KO , with serine, a key amino acid induced by the mitochondrial stress response, remaining unchanged upon ACT treatment, and cysteine, important for glutathione synthesis, depleted from the media when compared to WT cells treated with ACT (Fig

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Cell debris was precipitated by adding 150 L of Tail Salts Buffer (4.31 M NaCl, 0.63 M HCl, 10 mM TrisHCl, pH7.4) then samples were centrifuged at ~ 21,000 g for 15 min at 4 C

the method comprises administering to a human in need of such treatment a pharmaceutical composition comprising a cyclobenzaprine analog disclosed herein in a therapeutically effective amount and a therapeutically acceptable carrier