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Cells were incubated overnight at 4 C with anti-YAP D8H1X antibody (14074, cell Signaling, 1/100 dilution) in 1% BSA and 0.3% Triton X-100 followed by incubation for 1.5 h at room temperature with goat anti-rabbit Alexa fluor 594 (A-11012, Invitrogen, 1/500 dilution)

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In addition, chronic consumption of red meat increased TMAO levels produced from carnitine, but not choline, and decreased renal TMAO excretion, and plasma TMAO levels decreased within 4 weeks after discontinuation of red meat consumption (Wang et al., 2019)

1 LPA also induces C-fiber retraction, A-fiber demyelination, and dorsal root fiber sprouting, contributing to allodynia and synaptic reorganization pain