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Treatment of the second aliquot with TCEP and IAM, followed by measurement of CAM-S-CAM-TPP after protein precipitation, gave the total amount of RSS /RS(S) n S present in the tissue homogenate, giving values that are consistent with earlier reports of overall tissue levels of persulfides using sulfane sulfur atom trapping 31,32

Mihaylova et al

Andrew Huberman: What are some cold protocols that you find particularly interesting or attractive from the standpoint of, I don't know, pick your favorite, metabolism, neuro/mood effects, brown fat stimulation, which, of course, weaves back to metabolism

3.2.6 O-GlcNAcylation O-GlcNAcylation is another type of PTM, which involves the addition of N-acetylglucosamine (GlcNAc) to Ser or Thr residues (Figure 4D)