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Description

In industry, glutathione and reduced glutathione (GSH) are used as functional ingredients in pharmaceuticals, dietary supplements, cosmetics, and some food applications, where their antioxidant and reducing properties help stabilize formulations and support health-oriented product positioning

Figure 1 The upstream and downstream homologous arms of gr were amplified by PCR using primer pairs of gr UF/ gr UR [819 bp upstream homologous arm (UHA)] and gr DF/ gr DR [933 bp downstream homologous arm (DHA)]

10.1002/jmv.1890150110 40

The kanamycin cassette was removed from the resulting strains using the pCP20 helper plasmid [19] with the formation of the corresponding deletions ( cysB, cysE, gshA, gshB and emaA )