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High-resolution bisulfite-sequencing of peripheral blood DNA methylation in early-onset and familial risk breast cancer patients

Subsequent desulfurization using our optimized protocol 34 was not successful for the DAM27 ligation product, but an alternative protocol using 2-mercaptoethane sulfonate (MESNa) instead of glutathione resulted in quantitative conversion of cysteine to alanine giving rise to the native DAM27 monobody sequence (Figs

Flora Guerra, University of Salento, Italy Updates Copyright 2021 Li, van Dijk, Meeldijk, Kok, Blommestein, Verbakel, Kotte, Broekhuizen, Lacl, Goldschmeding, Cheng and Bovenschen

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