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Fluorescence ( ex = 405 and 488 nm, em = 510 nm) was measured by flow cytometry using a Becton Dickinsons FACS Aria II flow cytometer

Am J Physiol Endocrinol Metab (2005) 288(1):E1949.10.1152/ajpendo.00149.2004 177 NielsenCGormsenLCJessenNPedersenSBMollerNLundSet alGrowth hormone signaling in vivo in human muscle and adipose tissue: impact of insulin, substrate background, and growth hormone receptor blockade

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In these studies, Epithalon administration in elderly or immunosuppressed animals leads to increased production of thymic peptides and improved immunological parameters, including CD4/CD8 ratios and natural killer (NK) cell activity [3]