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This protocol is an extension of the widely accepted PEN-FAST (penicillin allergy reported by patient, five years or less since reaction, anaphylaxis or angioedema, severe cutaneous adverse reaction, and treatment required for reaction) clinical decision tool that has been used to identify patients with reported penicillin allergy who are appropriate for DOC rather than immediate hypersensitivity skin testing, which recent data suggest has poor positive predictive value in low-risk patients

doi: 10.1021/acssuschemeng.4c08602 78 NaczkMShahidiF

Immunocytochemistry Prior to the start of experiments, glass cover slips (Bellco Glass Inc., Vineland, NJ, USA) were coated in 24-well plates (Thermo Fisher Scientific) with PLO and FN before seeding the cells

Interdisciplinary collaboration among chemists, agronomists, data scientists, and policymakers is essential to develop harmonized regulatory frameworks with standardized global protocols and to support the global implementation of advanced residue monitoring systems