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Copper-Dependent Enzyme Activation GHK-Cu serves as a bioavailable copper delivery system, providing copper(II) ions to copper-dependent enzymes including superoxide dismutase (SOD), cytochrome c oxidase, and lysyl oxidase

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Finally, the purified enzyme was concentrated to approximately 200 M, aliquoted into 20 l volumes in PCR tubes, flash-frozen in liquid nitrogen, and stored at -80 C

2 Biosynthesis of melanin and its regulation 2.1 Melanin synthesis pathway The specific process of melanin synthesis begins with the conversion of tyrosine into L-Dopa catalysed by tyrosinase, the central glycoprotease in the melanosome region and the only rate-limiting enzyme in this process (Kim et al., 2023)