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Supernatant was centrifuged at 16,000 g for 15 min at 4 C to remove nuclei and membranes

Nrf2 is critical for cellular protection against oxidative stress, and NOX4, closely linked to the TGF-1/Smad3 pathway in lung fibroblasts, is essential for regulating the myofibroblast phenotype in IPF

Based on the optimisation steps, the mobile phase, which consisted of (A) 80 mM TFA and (B) 100% methanol, was used for separation and detection of GSH and GSSG