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Surprisingly, at the SC- cell stage (day 21), we did not observe significant changes in the protein levels of key cell markers between WT and GPX2 KO cells, including PDX1, CHGA 44 , NKX6-1 45 , and C-PEP 46 (Fig

the samples were then cleaned with distilled water, fixed in 3% glutaraldehyde, rinsed in phosphate buffer, and post-fixed in potassium permanganate solution for five minutes