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Animals under anesthesia were then transferred to a nose cone and continued to receive 15% isoflurane at an O 2 set to 1.0 L/min and then exsanguinated by cardiac puncture using a 25 gauge needle followed by cervical dislocation

In addition, 41 genes were triggered in endomembrane system components with an FDR score of 2.69 10 5 (Supplementary Table 7

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