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The supernatant must be diluted 1:2.5 with Assay Buffer by mixing one part with 1.5 parts of Assay Buffer to bring the SSA concentration to 1 %

This protein is responsible for reabsorbing glucose back into the bloodstream, but SGLT2 inhibitors prevent this from happening, resulting in increased glucose excretion in the urine, which improves glycemic control in people with T2DM

The authors also acknowledge Hui-Ying Wang of the Public Platform of State Key Laboratory of State Key Laboratory of Natural Medicines at China Pharmaceutical University for the use of analytical instrumentation facilities