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Elution 3.1 Nucleic acid Elution Step: Add 50100 L of Elution Buffer I to the magnetic beads, incubate for 5 minutes at 65C or 2 minutes at 90C, place on a magnetic rack, perform magnetic separation and collect the supernatant

Genes Dev (2018) 32:94452

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In this work, we used a combination of biophysical techniques and cryo-electron microscopy (Cryo-EM), showing that human CBS full-length protein polymerises as a filament adopting two very different morphologies dependent on SAM binding and that polymerisation plays a role in both enzyme activation and stability