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10.3889/oamjms.2017.122 83 GicquiauxH.LecatS.GaireM.DieterlenA.MlyY.TakedaK.et al

For immunofluorescence staining, brain tissues were dissected out, post-fixed by 4% paraformaldehyde (PFA) for 12 h, embedded in paraffin, cut into 5 mm sections and mounted on slides for subsequent staining

Additional file 3

Meanwhile, human clinical data are generally characterized by small sample sizes and short follow-up periods, with inadequate adjustment for confounding factors such as diet and concurrent medications, making it difficult to confirm the long-term efficacy and safety of Clostridium interventions