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Briefly, 50100 l of saturated culture was spun down and cells were thoroughly resuspended in 100200 l of lysing buffer (62.5 mM Tris-HCl, pH6.8, 10% glycerol, 2% SDS, 4% -mercaptoethanol)

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In particular, miR-9 downregulation due to increased glucose concentration, was associated with increased cell proliferation, epithelial-to-mesenchymal transition (EMT), migration and invasion

Cell proliferation and interleukin-6-type cytokine signaling are implicated by gene expression responses in early optic nerve head injury in rat glaucoma