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This belief significantly influenced the determination of the mRNA doses in these genetic vaccines, and it is an extension of the observation that survivors of severe COVID-19 exhibited a survival advantage by rapidly generating higher levels of neutralizing antibodies compared to binding (non-neutralizing) antibodies, unlike non-survivors, who produced these antibodies more slowly and at lower levels

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A 24 h time point was chosen based on a previous report of using this time period for transfecting or treating 3T3-L1 cells with NNMT anti-sense oligonucleotides or a small molecule NNMT product inhibitor (1-MNA), respectively, for phenotypic measures

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