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Rather than assessing the overall protein adduct formation in the cell, which was initially considered to be critical for cell death20, it now appears that protein adduct formation on mitochondrial proteins is most relevant for toxicity2123

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of residual GSH in the test tube)/(10 min) * [(Total volume (ml)/ Volume of sample (ml)] * D.F (2) Standard curve To establish a standard curve for the modified GPx-DTNB method, we diluted the glutathione standard with 0.1 M phosphate buffer (pH 7.0) to create a 5 to 500 M concentration range, as shown in Fig