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We recommend blocking free thiols with alkylating agents like NEM or IAM during protein extraction to stabilize the modification

Mallozzi, C., Di Stasi, M

For cell scratch assay, HaCaT cells (13 1e6) or human keratinocyte primary cultures (5 1e5) were seeded in six/twelve-well plates with Dulbeccos modified Eagles medium (DMEM) containing 5% heat-inactivated FBS or with dermal cell basal medium supplemented with keratinocyte growth kit components (obtained from ATCC, USA)

The recommended daily dose of glutathione is 250-500 mg, which is generally regarded as safe, with possible mild side effects like bloating or cramping