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The authors also showed that reduced methylation in key cytokine genes such as IL4 , IL5 , IL13 , IL12B and IL17F was associated with increased disease activity, suggesting an epigenetic shift towards a Th2 and possibly Th17 response

Further, Western blotting was employed to evaluate the expression of Akt, PI3K, and nuclear factor-B (NF-B) p65

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