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To minimize the batch effects and to ensure that all sections receive the same downstream treatment, sections were mounted in a way that all analyzed samples (all WT bio-replicates and all nifH- bio-replicates) were on the same ITO slide, analogous to well-established tissue microarray (TMA) clinical assays (Grzeski et al., 2022)

doi: 10.1007/978-3-319-20825-1_6 177 Osuna-RamosJFCamberos-BarrazaJTorres-MondragonLERabago-MonzonARCamacho-ZamoraAValdez-FloresMAet al

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The condition is the most common form of neurofibromatosis and occurs in about 1 in 3,000 births worldwide each year