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PC compositional change increases the open probability of RyR and potential Ca 2+ leakage from the sarcoplasmic reticulum To identify the molecular mechanisms underlying the decreased contractile force of skeletal muscle in Gde5 skKO mice, we isolated single muscle fibers from the gastrocnemius (GAS) using fine forceps, and a segment of the skinned fiber was used to analyze the myofibril and sarcoplasmic reticulum (SR) properties (Figs

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