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The cells in a 96-well plate were incubated in HBSS containing 10 mM FAO blue for 2 h, washed twice with HBSS, and measured using a Spectramax M2 plate reader (excitation/emission = 405 nm/465 nm)

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Mullikin D, Pillai N, Sanchez R et al (2018) Megaloblastic anemia progressing to severe thrombotic microangiopathy in patients with disordered vitamin B12 metabolism: case reports and literature review

Among the most potent of these lipid species are lysophosphatidic acids (LPAs) consisting of a phosphate head group attached to a glycerol backbone and a single FA tail