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Immunofixation interference Furthermore, administered human IgG1, monoclonal antibodies can interfere with the detection of clonal IgG, molecules in the sera of MM patients, as measured by serum protein electrophoresis and/or immunofixation electrophoresis (IFE), owing to comigration in the assays [53]

If you massage it or put a warm flannel it should disperses any b12 pooled there The blue is most likely a bruise from the bleed

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