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To investigate the function of CBS in this process, we silenced its expression by CRISPR/cas9 mediated gene knockout strategy (sg CBS ) in SCD erythroblasts (Fig

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For example, charcoal adsorbs toxins as opposed to tightly binding toxins

Next, denatured protein samples (15 g per lane, diluted in 5 loading buffer and boiled at 95 C for 5 min) were separated on 10% SDS-PAGE gels and transferred to PVDF membranes (Millipore, USA) using a Pyxis Gel Processor (China)