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Description

[PMID:25974072] Calabro KA, Harmon CM, Vali K

Western blot Pools of two retinas were homogenized manually with RIPA buffer (containing 150 mM of NaCl, 1% NP 40, 0.5% of Na deoxycholate, 0.1% of SDS (sodium dodecyl sulfate) and 50 mM of Tris at pH 8)

Funders did not influence the content of the study

Plates were subsequently washed with 0.1% polyethylene sorbitan monolaurate (Tween20)-Tris-buffered saline (TTBS), immediately fixed with cold 3.7% paraformaldehyde in PBS, and re-treated with the blocking solution overnight at 4C