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This effect was not seen when the DHHS-mutant of zDHHC5 was used, suggesting that GCP16/Golga7 is a substrate for zDHHC5 (Supplementary Fig

AB staining identified sperm cells with abnormal chromatin packaging (dark blue nuclei), whereas AO staining distinguished between spermatozoa with normal DNA (green) and damaged DNA (orange/red) 99

These cascades involve the activation of NF-B and MAPK pathways, upregulation of inflammatory cytokines, and overproduction of reactive oxygen species (ROS), collectively accelerating cellular senescence and tissue degradation 8,9,10,11,12