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Alexa Fluor 488 Annexin V/Dead cell apoptosis assay Dead cell apoptosis assay is quick and suitable method for identification of apoptotic cells as Annexin V, being phospholipid binding protein has more affinity towards phosphatidyl serine, which moves from inner plasma membrane leaflet to outer leaflet in apoptotic cells and thereby these apoptotic cells can be quantitatively estimated [40] For this assay, Alexa Fluor 488 Annexin V/Dead cell apoptosis kit (Thermo Fischer Scientific, USA) was used

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The enhanced susceptibility of metZ to Pyrazinamide (PZA), a prodrug that gets activated upon exposure to acidic pH, further confirms the presence of a relatively acidic cytosol and accentuates the role of metZ in maintaining the pH homeostasis in M

doi: 10.1038/s41467-019-08680-6 182 WangC