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Zebrafish: an emerging model system for human disease and drug discovery

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10.1007/BF01061422 36

Disease-anchored panels and how to act on them For disease-anchored panels, tailor the readouts to the biology and act on the map they provide: in cardiovascular/vascular beds, pair DHE-HPLC (to quantify O 2 ) with compartment-targeted HyPer for H 2 O 2 , then add F 2 -isoprostanes/3-nitrotyrosine and iron indices to distinguish NOX-driven endothelial dysfunction (O 2 ONOO lowering NO bioavailability) from mitochondrial bursts typical of ischemiareperfusion (Bkgaard Nielsen et al