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If you prefer injecting IM, follow the same instructions

By making this substitution at position three, IGF-1 is inhibited from binding to certain proteins in the body, thus increasing the half-life ( Effects of IGF-1 on Growth Hormone Secretion IGF-1 is a peptide that acts similarly to insulin, and its main function in the body is to stimulate growth by increasing GH secretion from the pituitary gland, as well as regulating cell growth and cell death

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The ligation mixture is then used to transform bacteria HB101, which are then plated onto agar containing kanamycin for the purpose of confirming that the vector has the gene of interest properly inserted