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Co-Targeting Intrinsic and Extrinsic Apoptosis to Maximize Cell Death Induction in Venetoclax-Resistant AML Cells

Immunofluorescence staining HFF-1 cells (1.5 10 5 cells per well) or mouse embryonic fibroblasts (NIH3T3) were grown on sterile cell slides for 24 h till 80% confluence

Treatment Compositions [00043] A first disclosed compositions for removing the off-odor from an aqueous solution of glutathione by a redox reaction and the addition of 1,3,5-Triazine-1,3,5(2H, 4H,6H)-triethanol

After parasite pellet resuspension in SDS lysis buffer (50 mm Tris-HCl pH 8, 10 mm EDTA pH 8, 1% SDS), protein quantification was performed with a bicinchoninic acid assay kit (Abcam) and for each condition, 20 g of total proteins were separated on a 12% SDS-PAGE run for 20 min at 100 V, stained with colloidal blue (Thermo Fisher Scientific)