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Following strict storage guidelines helps preserve structural integrity

Preparation of Tissue Homogenate Two brain tissue homogenates were prepared, one in cold buffer (phosphate buffer saline, pH 7.4) in the ratio (1:10 w/v ) for the assay of GPX4, IL-1, TfR1 and ferritin and another in (50 mM potassium phosphate, pH 7.5) in the ratio (1:10 w/v ) for the assay of MDA, using tissue homogenizer (WiseTis homogenizer HG-15D Witeg Labortechnik GmbH, Germany)