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Studies have shown that reduced glutathione presented in tumor cells' cytoplasma is up to 1000-fold higher than that present in normal cells' cytoplasma, and the tumor cells also contain enzymes of the protein disulfide isomerase family, which may contribute to reduction of the disulfide bond in cellular compartments

Therefore, it is crucial to determine the dynamics, quantity, size, and morphology of -H2AX-related foci to distinguish them from actual DNA damage

For instance, mutation of TTLL1 causes male infertility, massive defects in the assembly of the sperm flagellum, defects in the function of the nasal (Vogel et al, 2010), and airway ciliated epithelia (Ikegami et al, 2010)