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Consequently, the transformation of LC3-I to the autophagosome-associated form LC3-II is weakened, thereby reducing Beclin-1 and autophagy-related gene 5 expression and decreasing autophagy vesicle formation [129]

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doi: 10.3390/biomedicines13092190 91 ChisariMGEspositoMAllocaSVarrialeBSalernoMRasoGMet al

This also reflects the advantage of utilizing mustard oils against insect pests as they are easy and economical to produce as compared to AITC which is a highly costly and purified metabolic product synthesized industrially, though practical application faces significant formulation challenges including volatility, oxidation susceptibility, and the need for sophisticated stabilization technologies that could substantially increase production costs